Proteomics is the study of the entire set of proteins produced in an organism, system, or biological situation. Compared to DNA or transcripts, proteins are directly linked to phenotypic behaviour. Therefore, expression of proteins is considered a better measure of cellular activity and its comparative analysis often used to obtain valuable information across different cellular states, diseases, or organisms. We employ LC-MS/MS based label free quantitative proteomics to elucidate the molecular mechanism for various biological contexts.