N-glycans analysis workflow at NFML
N-glycans analysis workflow at NFML

글리코실화(탄수화물이 거대분자에 부착되는 현상)는 다양한 생체 분자의 기능을 조절하는 데 중요한 역할을 합니다. 글리코실화는 분자가 각각의 기능을 수행하는 방식에 영향을 미치고 음식은 유리 탄수화물과 당접합체를 모두 포함하기 때문에 주요 생물학적 시스템에서 유리 탄수화물 뿐만 아니라 글리칸 잔기의 유형, 구조 및 양을 밝히는 것은 영양, 질병 진단, 모니터링 및 치료에 큰 의미가 있습니다. NFML은 LC MS/MS 및 글리코정보학 접근법을 모두 사용하여 다양한 바이오 샘플에서 글리칸을 식별, 특성화 및 정량화합니다. 이에 대한 우리의 주요 초점은 예후 및 진단 바이오마커를 모두 발견하는 것이고 다양한 시스템과 유기체에 걸쳐 글리코실화가 세포 기능에 미치는 영향에 대한 중요한 생물학적 정보를 제공하는 다양한 생체 재료의 글리칸을 프로파일링하고 특성화하기 위해 노력해 왔습니다. 이에 대한 우리의 주요 협력자는 AGRS입니다.

연구 결과

  1. In-depth characterization of non-human sialic acid (Neu5Gc) in human serum using label-free ZIC-HILIC/MRM-MS. Analytical and Bioanalytical Chemistry 2021, 413, 5227-5237.
  2. Isomer-Specific Monitoring of Sialylated N-Glycans Reveals Association of α2,3-Linked Sialic Acid Epitope with Behcet’s Disease. Frontiers in Molecular Biosciences 2021, 1120.
  3. Detection of Aberrant Glycosylation of Serum Haptoglobin for Gastric Cancer Diagnosis Using a Middle-Up-Down Glycoproteome Platform. Journal of Personalized medicine 2021, 11(6), 575.
  4. Spatial and temporal diversity of glycome expression in mammalian brain. Proceedings of the National Academy of Sciences, 2020, 117.46: 28743-28753.
  5. Discovery of N-glycan Biomarkers for the Canine Osteoarthritis. Life (Basel) 2020, 10(9)
  6. Glycosylation of serum haptoglobin as a marker of gastric cancer: an overview for clinicians. Expert Review of Proteomics 2020, 17(2):109-117.
  7. Glycomic profiling of targeted serum haptoglobin for gastric cancer using nano LC/MS and LC/MS/MS. Molecular Biosystems 2016, 12(12):3611-3621.
  8. Mass Spectrometric Screening of Ovarian Cancer with Serum Glycans. Disease Markers, pp. 1–9.
  9. Designation of fingerprint glycopeptides for targeted glycoproteomic analysis of serum haptoglobin: insights into gastric cancer biomarker discovery. Analytical and bioanalytical chemistry 2018, 410(6):1617-1629.
  10. Direct analysis of aberrant glycosylation on haptoglobin in patients with gastric cancer. Oncotarget 2017, 8(7):11094-11104.
  11. Integrated GlycoProteome Analyzer (I-GPA) for Automated Identification and Quantitation of Site-Specific N-Glycosylation. Scientific  Reports 2016, 6:21175.
  12. Analytical detection and characterization of biopharmaceutical glycosylation by MS. Bioanalysis 2016, 8(7):711-727.
  13. Glycosylated proteins preserved over millennia: N-glycan analysis of Tyrolean Iceman, Scythian Princess and Warrior. Scientific Reports 2014, 4:4963.
  14. Serum glycan signatures of gastric cancer. Cancer Prevention Research (Phila) 2014, 7(2):226-235.
  15. Mass spectrometric screening of ovarian cancer with serum glycans. Disease Markers 2014, 2014:634289/634289. 
  16. Differentiation of cancer cell origin and molecular subtype by plasma membrane N-glycan profiling. Journal of Proteome Research 2014, 13, 2, 961–968.
  17. Characterization of novel O-glycans isolated from tear and saliva of ocular rosacea patients.Journal of Proteome Research 2013, 12(3):1090–1100.

  18. The Fifth ACGG-DB Meeting Report: Towards an International Glycan Structure Repository. Glycobiology 2013, 23(12):1422–1424.
  19. The Year of Glycosciences. Glycobiology 2013, 23(2):143–146.
  20. Glycomic analysis of tear and saliva in ocular rosacea patients: the search for a biomarker. Ocular Surface 2012, 10(3):184-192. 
  21. Extensive determination of glycan heterogeneity reveals an unusual abundance of high mannose glycans in enriched plasma membranes of human embryonic stem cells. Molecular & Cellular Proteomics 2012, 11(4):1–13.
  22. Evolutionary glycomics: characterization of milk oligosaccharides in primates. Journal of Proteome Research 2011, 10(4):1548–1557.
  23. High-mannose glycans are elevated during breast cancer progression. Molecular & Cellular Proteomics 2011, 10(1):1–9.
  24. Glycomic approach for potential biomarkers on prostate cancer: profiling of N-linked glycans in human sera and pRNS cell lines. Disease Markers 2008, 25(4-5):243-258.